Characterization of microbial communities
Characterization of microbial communities
However, a prerequisite realizing the full potential of microbial communities is to be able to identify the key organisms. The current approach for microbial identification is rRNA amplicon sequencing, but this method has several severe biases and drawbacks that need to be solved. In this project the aim is to overcome these limitations by the development of novel methods and apply these for an unbiased view of microbial communities.
Funding
The project is funded by The Danish Council for Independent Research (FNU) in the period 2016-2019.
Project content
- Development of primer-free full-length 16S rRNA sequencing
- Establish a comprehensive full-length 16S rRNA databases environmental Biotechnology
- Re-design of primers and high-throughput screening of ecosystems in Environmental Biotechnology.
- Re-design of FISH probes and in situ investigations
Project participants
Aalborg University, Center for Microbial Communities:
- Per Halkjær Nielsen, Professor (project leader)
- Jannie Munk Kristensen, PhD student
- Mads Albersen, Professor
- Morten Dueholm, Assistant Professor
- Simon McIlroy, Post.Doc.
- Marta Nierychlo, Post.doc.
Collaboration
- Professor Dr. Michael Wagner, Department of Microbial Ecology, Univ. of Vienna